Paket Lengkap Antioxidant Activity Of Combination Ethanol Extract Of Turmeric Rhizome (Curcuma Domestica Val) And Ethanol Extract Of Trengguli Bark (Cassia Fistula L) With Dpph Method

Abstrak: Turmeric rhizome (Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome - trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL and 41,95 μg/mL, respectively.

Keywords: Antioxidant, Curcuma domestica, Cassia fistula, DPPH Method

Penulis: Triyono, Anis Yohana Chaerunisaa, Anas Subarnas

Kode Jurnal: jpfarmasidd180222